double-stranded RNA polymerase promoter region in the correct orientation. Consensus promoter sequences of different RNA Polymerases: T7 TAATACGACTCACTATAGGG T3 AATTAACCCTCACTAAAGGG SP6 ATTTAGGTGACACTATAGAA G will be the first base (+1) of the RNA transcript The synthesis of sense or antisense RNA transcripts Restriction fragments of T7 DNA which selectively bind E. coli RNA polymerase have been identified. These include fragments located close to the beginning of gene 1 where according to Minkley and Pribnow (1973) there is a promoter called C. The smallest fragment from this region which binds RNA polymerase has been sequenced.
T7 phage RNA polymerase has a high specificity for its respective promoter. Once T7 RNA polymerase binds to its double-stranded DNA promoter, it separates the two DNA strands, and uses the 3' to 5' strand as a template to synthesize a complementary strand at the end of the DNA template (run-off transcription) (Figure 1).

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We have determined the nucleotide sequence of a Hpa II restriction fragment of the phage T7 DNA containing a promoter for the phage-specified RNA polymerase. (Hpa II is a restriction endonuclease from Haemophilus parainfluenzae.) Mapping of the Hpa II restriction fragments on the T7 genome shows this promoter to be the second of tandem promoters separated by approximately 170 base pairs that ... -
We have determined the nucleotide sequence of a Hpa II restriction fragment of the phage T7 DNA containing a promoter for the phage-specified RNA polymerase. (Hpa II is a restriction endonuclease from Haemophilus parainfluenzae.) Mapping of the Hpa II restriction fragments on the T7 genome shows this promoter to be the second of tandem promoters separated by approximately 170 base pairs that ...
Jun 20, 2009 · In vitro T7 transcription is the synthesis of RNAs using a T7 promoter and purified enzyme. It is the standard method of making up to several mg of RNAs longer than about 20nts with relatively high quality as compared to solid phase synthesis.

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The T7, T3 and SP6 RNA polymerases are a homologous family of enzymes that recognize and discriminate among similarly homologous but different promoter sequences. -
Under conditions where cycling is minimal (wild-type polymerase, gene10 ITS), T7 promoter drives the synthesis of three long transcripts per second at 37°C in vivo, a figure higher than for any Escherichia coli promoter. KW - Abortive transcription. KW - Promoter strength. KW - T7 RNA polymerase. KW - Transcription processivity
double-stranded RNA polymerase promoter region in the correct orientation. Consensus promoter sequences of different RNA Polymerases: T7 TAATACGACTCACTATAGGG T3 AATTAACCCTCACTAAAGGG SP6 ATTTAGGTGACACTATAGAA G will be the first base (+1) of the RNA transcript The synthesis of sense or antisense RNA transcripts

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Under conditions where cycling is minimal (wild-type polymerase, gene10 ITS), T7 promoter drives the synthesis of three long transcripts per second at 37°C in vivo, a figure higher than for any Escherichia coli promoter. KW - Abortive transcription. KW - Promoter strength. KW - T7 RNA polymerase. KW - Transcription processivity -
T7 RNA polymerase is also highly selective for initiation at its own promoter sequences and is resistant to antibiotics such as rifampicin that inhibit E. coli RNA polymerase.
double-stranded RNA polymerase promoter region in the correct orientation. Consensus promoter sequences of different RNA Polymerases: T7 TAATACGACTCACTATAGGG T3 AATTAACCCTCACTAAAGGG SP6 ATTTAGGTGACACTATAGAA G will be the first base (+1) of the RNA transcript The synthesis of sense or antisense RNA transcripts

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One (the expression vector) contains p (T7) upstream of the gene to be expressed. The second contains the T7 RNA polymerase gene under the control of a heat-inducible E. coli promoter. Upon heat induction, the T7 RNA polymerase is produced and initiates transcription on the expression vector, resulting in turn in the expression of the gene (s) under the control of p (T7). -
In this vector, MDR1 is controlled by the T7-promoter (TTP) and the presence of an IRES sequence between the T7-promoter and MDR1 sequence facilitates cap-independent translation on transfection of cells infected with vTF 7-3, a recombi- nant vaccinia virus encoding T7 RNA polymerase.

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The structure of a T7 RNA polymerase (T7 RNAP) initiation complex captured transcribing a trinucleotide of RNA from a 17–base pair promoter DNA containing a 5-nucleotide single-strand template extension was determined at a resolution of 2.4 angstroms. Binding of the upstream duplex portion of the promoter occurs in the same manner as that in the open promoter complex, but the single-stranded ... -
Description: T7 RNA Polymerase is a DNA-dependent RNA polymerase derived from the T7 bacteriophage which exhibits a high recognition specificity to the T7 promoter and terminator sequences and catalyzes the 5' -> 3' synthesis of RNA starting at a T7 promoter sequence (1,2).
T7 Polymerase. This enzyme derives from T7 phage. It requires double-stranded DNA that contains the T7 promoter sequence as a template and NTPs as substrates and synthesizes the single-strand RNA was complementary to the template downstream of the promoter.

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Sequence. Modern lacUV5 is seen in the BL21(DE3) strain, which carries both a lac operon with the standard promoter and a lacUV5 operon split by the DE3 prophage (and as a result driving the T7 RNA polymerase instead). The two important mutations are underlined. -
Bacteriophage T7 promoters contain a consensus sequence from -17 to +6 relative to the transcription start site, +1. In addition, the strong class III promoters are characterized by an extended AT-rich region upstream of -17, which is often interrupted by one or more GC base pairs in the weaker class II promoters.
Description: T7 RNA Polymerase is a DNA-dependent RNA polymerase derived from the T7 bacteriophage which exhibits a high recognition specificity to the T7 promoter and terminator sequences and catalyzes the 5' -> 3' synthesis of RNA starting at a T7 promoter sequence (1,2).

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Bacteriophage T7 promoters contain a consensus sequence from -17 to +6 relative to the transcription start site, +1. In addition, the strong class III promoters are characterized by an extended AT-rich region upstream of -17, which is often interrupted by one or more GC base pairs in the weaker class II promoters. -
Under conditions where cycling is minimal (wild-type polymerase, gene10 ITS), T7 promoter drives the synthesis of three long transcripts per second at 37°C in vivo, a figure higher than for any Escherichia coli promoter. KW - Abortive transcription. KW - Promoter strength. KW - T7 RNA polymerase. KW - Transcription processivity
• Functionally tested by dideoxy sequencing of a vector containing an appropriate RNA polymerase promoter sequence. Catalog and Promoter sequences: • SO118—T7 promoter sequencing primer, 20-mer 5'-d (TAATACGACTCACTATAGGG)-3' Note • Primers cannot be used for certain plasmids that contain truncated, but still fully functional promoters.

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RNA polymerase will bind to this core promoter region stably and transcription of the template strand can initiate. The TATA box is a DNA sequence (5'-TATAAA-3') within the core promoter region where general transcription factor proteins and histones can bind. Histones are proteins found in eukaryotic cells that package DNA into nucleosomes.

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To obtain a PCR product with a T7 promoter sequence incorporated in it at 3', the reverse primer should be added with a T7 sequence. By addition of such sequence the reverse primer becomes around... -
Jun 20, 2009 · In vitro T7 transcription is the synthesis of RNAs using a T7 promoter and purified enzyme. It is the standard method of making up to several mg of RNAs longer than about 20nts with relatively high quality as compared to solid phase synthesis.
Sequence. Modern lacUV5 is seen in the BL21(DE3) strain, which carries both a lac operon with the standard promoter and a lacUV5 operon split by the DE3 prophage (and as a result driving the T7 RNA polymerase instead). The two important mutations are underlined.

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RNA polymerase pulls most of the T7 genome into the newly infected cell (Zavriev and Shemyakin, 1982; Garcia and Molineux, 1995). Polymerase-mediated genome entry is a relatively slow process that results in the direct physical coupling of gene expression dynamics to gene position. -
Hi-T7 RNA Polymerase is an engineered DNA-dependent RNA polymerase that is highly specific for T7 phage promoters and is designed to function at higher temperatures than the wild-type bacteriophage T7 RNA Polymerase. Hi-T7 RNA Polymerase functions at an optimal temperature of 50-52°C. Product Source An E. coli strain that carries a plasmid ...

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Bacteriophage T7 promoters contain a consensus sequence from -17 to +6 relative to the transcription start site, +1. In addition, the strong class III promoters are characterized by an extended AT-rich region upstream of -17, which is often interrupted by one or more GC base pairs in the weaker class II promoters. -
Hi-T7 RNA Polymerase is an engineered DNA-dependent RNA polymerase that is highly specific for T7 phage promoters and is designed to function at higher temperatures than the wild-type bacteriophage T7 RNA Polymerase. Hi-T7 RNA Polymerase functions at an optimal temperature of 50-52°C. Product Source An E. coli strain that carries a plasmid ...
T7 phage RNA polymerase has a high specificity for its respective promoter. Once T7 RNA polymerase binds to its double-stranded DNA promoter, it separates the two DNA strands, and uses the 3' to 5' strand as a template to synthesize a complementary strand at the end of the DNA template (run-off transcription) (Figure 1).

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T7 RNA Polymerase is a DNA-dependent RNA poly-merase present in E. coliinfected with bacterio- phage T7 (1). The enzyme has extremely high speci-ficity for promoter sequences found in T7 bacterio- -
The 5' end of each primer can correspond to either the 27 nt “T7 promoter sequence” (TAATACGACTCACTATAGGGAGACCAC) [Carthew] or the 27 nt “T7 RNA polymerase binding site” (GAATTAATACGACTCACTATAGGGAGA) [Clemens]. Thus, each primer will be 47 to 51 nt long (27 + [20 to 24]).

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• Functionally tested by dideoxy sequencing of a vector containing an appropriate RNA polymerase promoter sequence. Catalog and Promoter sequences: • SO118—T7 promoter sequencing primer, 20-mer 5'-d (TAATACGACTCACTATAGGG)-3' Note • Primers cannot be used for certain plasmids that contain truncated, but still fully functional promoters. -
The structure of a T7 RNA polymerase (T7 RNAP) initiation complex captured transcribing a trinucleotide of RNA from a 17–base pair promoter DNA containing a 5-nucleotide single-strand template extension was determined at a resolution of 2.4 angstroms. Binding of the upstream duplex portion of the promoter occurs in the same manner as that in the open promoter complex, but the single-stranded ...
The 5' end of each primer can correspond to either the 27 nt “T7 promoter sequence” (TAATACGACTCACTATAGGGAGACCAC) [Carthew] or the 27 nt “T7 RNA polymerase binding site” (GAATTAATACGACTCACTATAGGGAGA) [Clemens]. Thus, each primer will be 47 to 51 nt long (27 + [20 to 24]).

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Highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. Recognizes a specific promoter sequence and enters first into an 'abortive phase' where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. -
T7 RNA polymerase is also highly selective for initiation at its own promoter sequences and is resistant to antibiotics such as rifampicin that inhibit E. coli RNA polymerase.

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In this vector, MDR1 is controlled by the T7-promoter (TTP) and the presence of an IRES sequence between the T7-promoter and MDR1 sequence facilitates cap-independent translation on transfection of cells infected with vTF 7-3, a recombi- nant vaccinia virus encoding T7 RNA polymerase.